attune flow cytometer - An Overview
attune flow cytometer - An Overview
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Information-enriched characterization of cell populations. Morphological facts from images can insert for the richness of flow cytometry knowledge. As an example, the determine exhibits an if not regular apoptosis assay employing Annexin V and PI, adding mobile imaging to characterize cells in Each individual populace to expose morphologically distinctive attributes. These insights could not are actually acquired from multiplex staining alone.
No matter if you’re a novice or a professional, explore our sources to progress your study and abilities in flow cytometry.
Incorporate acoustic concentrating engineering with large-parameter Evaluation for adaptable spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a large-velocity brightfield camera for simultaneous superior throughput flow cytometry and large resolution brightfield imaging.
"A picture isn't pretty value one thousand dots, but it surely really helps to see photographs. It is great to check out photos that correlate with staining. Such as, AnnexinV-positive cells that happen to be morphologically diverse than balanced cells, or CD14+ monocytes which might be greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a qualified consumer has gated singlets confidently. Immediately after analyzing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate consists of over 4% aggregates.
Optimizing gating system. Even sturdy guide singlet gating is error-vulnerable and continues to be a subjective decision point in almost all flow cytometry assays. Imaging can be employed to verify and modify gates to incorporate only one cells of interest.
Our researchers will style panels for yourself as many times you need—free of charge, no product obtain essential.
Early indications of this contamination had initially been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to find intriguing subpopulations that may not be apparent from flow cytometry facts on your own.
Perhaps most importantly, these gatherings incorporate cells of Plainly distinct phenotypes which may lead to incorrect conclusions with regards to double positive occasions (specifically in scarce populations).
AI-pushed automated picture Examination with the Attune Cytometric Software interprets celebration capabilities into distinctive morphology parameters which might be coupled with standard fluorescence and scatter parameters to reveal extremely reproducible inhabitants alterations and unique subsets.
Software note: Automated impression Assessment lowers consumer-to-person variability in flow cytometry gating methods
Leverage the power of State-of-the-art technological know-how and innovation to speed up your discoveries. Rely on inside the Attune Flow Cytometer loved ones to provide the dependability, velocity, and accuracy that set your get the job done apart.
No clean/no lyse leukocyte Examination with imaging. Cells have been acquired from 24-hour-old blood by dilution in one mM EDTA ( Skilled UX/UI allows buyers to teach AI designs for expanded programs. (A) The “Practice Design” attribute presents annotation equipment and real-time product instruction precision.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technologies, which makes use of sound waves to meticulously align cells inside the flow cell. This impressive technique surpasses common hydrodynamic techniques, leading to more quickly processing speeds, Remarkable details quality, and greater sample throughput.
The Attune Xenith cytometer is a higher-parameter spectral analyzer, giving equally spectral unmixing and conventional flow capabilities for in depth and multipurpose mobile Examination.
Agilent Worth Assure assures you at least 10 years of instrument use from your day you purchase. Normally, we will credit you Using the residual price of the technique toward an upgraded product.
What appeared to be a homogeneous inhabitants is definitely additional diverse—an insight here that needs to be considered when interpreting benefits.